First documented use of fetal membranes Dr Roth 1940
in patients with symblepharon.
1946 : Sorsby and Symons: To treat eyes with chemical burns.
1993 : Batle and Perdotno use as a substitute for repair of
conjunctival defects and reconstruction fornices
Dry amniotic membrane is packaged in a sterile
pouch. Tear the outer pouch and pass the inner pouch into a sterile field.
Remove the tissue from the inner pouch using sterile gloves/ forceps. Apply
dry amniotic membrane directly to the patient site. If required, the
membrane can be hydrated with sterile saline or sterile water. Once the
inner pouch is opened, membrane should be preferably used immediately.
How To Use Am (Amnio Cornea-GraftTM)
is cut into corresponding size
and shape slightly larger than the size of the defect and placed on the
defect with the basement side facing up. It is usually sutured on to the
cornea. The stitching becomes easy if the membrane stitched in its dry form
and then wetted. Now a days the membrane can be glued to the site with
fibrin glue and there is no need of stitches, and post operative irritation,
watering, pain and the surgery time is much reduced.
- Promoting epithelialization
- Inhibiting inflammation and fibrosis.
The basement membrane of the AM (Amnio Cornea-GraftTM)
and conjunctiva are very similar. This promotes epithelial cell
differentiation, facilatates adhesion of basal epithelial cells and promote
epithelial cell differentiation.
AM (Amnio Cornea-GraftTM)
also offers mechanical
support and promotes epithelial healing as it gets hydrated by tears and
provides hydration to the regenerating epithelium